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47,48 Glucosidases I and II in the ER progressively remove glucose residues from the N -glycans of newly synthesized glycoproteins to generate Glc 1 Man 9 GlcNAc 2 , which is detected by the calnexin/calreticulin cycle and serves as a signal to promote glycoprotein folding

Refrigeration is appropriate for shorter storage, while -20 C freezer storage is the conservative long-term approach when supplier instructions allow it

4 The MTC contraindication is a precaution based on animal data, not evidence of harm in humans

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